MassIVE MSV000086651

Partial Public PXD023327

DNA damage-induced alternative splicing of p53

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Description

Samples were reduced in loading buffer and separated by SDS-PAGE for 3-4 min followed by in-gel digestion with trypsin. Digests were analyzed by LC-MS/MS using a Waters nanoACQUITY interfaced to a Thermo Fusion Lumos MS. The LC separation 90 min gradient of 5-30% MeCN in a trapping configurations with a 75 um x 25 cm analytical column (Waters HSS T3). MS used orbitrap MS1 and iontrap MS/MS (OT-IT). Raw files were converted to .mgf using Proteome Discoverer and searched against a Swissprot human database appended with p53beta sequence and containing reverse decoys, with fixed carbamidomethyl modification on Cys and variable deamidation (NQ), oxidation (M), peptide N-terminal Gln-->pyroGlu and protein N-terminal acetylation. Data was annotated at a 1% peptide and protein FDR using Scaffold. [doi:10.25345/C5678T] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: p53 ; BioID ; BirA ; GFP

Contact

Principal Investigators:
(in alphabetical order) 		Michael Kastan, M.D., Ph.D., Duke University, USA
Submitting User: mwfoster

Publications

Chen J, Zhang D, Qin X, Owzar K, McCann JJ, Kastan MB.
DNA-Damage-Induced Alternative Splicing of p53.
Cancers (Basel). Epub 2021 Jan 12.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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