This dataset submission includes the raw files used to sequence a mix of 5 human monoclonal antibodies as a benchmark proof-of-concept study for the manuscript "de novo Protein Sequencing of Antibodies for Identification of Neutralizing Antibodies in Human Plasma Post SARS-CoV-2 Vaccination". Refer to the attached metafile for detailed descriptions of the experiments. Briefly, bottom-up data was generated by performing in-solution digestions on the monoclonal antibodies on their own and after mixing to generate a pseudo-polyclonal mix. Separation was performed using native gel and non-reducing SDS-PAGE (NRT), followed by in-gel digestion for heavy-light chain pairing. Lastly, C-terminal modification and EThcD fragmentation of in-solution digests allowed for I/L determination.
[doi:10.25345/C58K7576M]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: covid19 ; de novo ; sequencing ; antibody ; neutralizing ; polyclonal
Principal Investigators: (in alphabetical order) |
Thierry Le Bihan, Rapid Novor, Canada |
Submitting User: | ChelseaReitzel |
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Owner | Reanalyses | |
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