Description
NKG7 is a lysosomal membrane protein whose expression is restricted to cytotoxic lymphocytes. Although the importance of NKG7 in anti-tumor immunity has been shown, little is known about the molecular function and regulation of NKG7. To elucidate NKG7 molecular function, we performed NKG7-proximity labeling using TurboID system in 293T cells, followed by mass-spectrometry. We identified vacuolar H+ ATPase (v-ATPase; lysosomal proton pump) subunit ATP6AP2 as a potential NKG7-interacting protein, and validated the interaction via exogenous coimmunoprecipitation. We further showed that NKG7 inhibits v-ATPase activity, thereby impacting lysosomal homeostasis and mTORC1 regulation at lysosomes.
[doi:10.25345/C5610W357]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: TurboID, proximity labeling, NKG7, cytotoxic lymphocytes, lysosome
Contact
Principal Investigators:
(in alphabetical order)
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Daniel Billadeau, Ph.D., Mayo Clinic, Rochester, MN, USA
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KLJohnson218
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Number of distinct conditions across all analyses (original submission and reanalyses)
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Distinct condition labels are counted across all files submitted in the "Metadata" category
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Number of distinct biological replicates across all analyses (original submission and reanalyses)
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Distinct replicate labels are counted across all files submitted in the "Metadata" category
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"N/A" means no results of this type were submitted.
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combination of condition and biological replicate was analyzed across all files submitted in the
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"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically
remapped by MassIVE to proteins in the
SwissProt
human reference database.
"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and
reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original
submission and reanalyses) associated with this dataset.
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Number of distinct peptide sequences (including modified variants or peptidoforms) reported
across all analyses (original submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all
analyses (original submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct proteins quantified across all analyses (original submission and reanalyses)
associated with this dataset.
Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
of Quantified Analytes" category having a "Protein" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison
across all analyses (original submission and reanalyses) associated with this dataset.
A protein is differentially abundant if its change in abundance across conditions is found
to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated
with statistical tests for differential abundance.
Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
of Quantified Analytes" category having a "Protein" column in this dataset.
"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE.
It has been imported to MassIVE for reanalysis purposes, so its spectra data here may
consist solely of processed peak lists suitable for reanalysis with most software.