MassIVE MSV000094898

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Longitudinal Analysis of the Lung Proteome Reveals Persistent Repair Months after Mild to Moderate COVID-19

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Description

In order to assess the restoration of homeostasis in the lung proteome after COVID-19 infection, we performed bronchoalveolar lavage on 45 patients with mild to moderate disease at three phases (acute, recovery, convalescence) over a year. Changes in proteins were assessed using a multimodal approach. During the acute phase, inflamed and uninflamed phenotypes were characterized by the expression of tissue repair and host defense response molecules. With recovery, inflammatory and fibrogenic mediators declined and clinical symptoms abated. However, at nine months, quantified radiographic abnormalities had resolved in the majority of patients, and yet compared to healthy persons, all showed ongoing activation of cellular repair processes and depression of the renin-kallikrein kinin-coagulation and complement systems. This dissociation of prolonged reparative processes from symptom and radiographic resolution suggests that occult ongoing disruption of the lung proteome is underrecognized and may be relevant to recovery from other serious viral pneumonias. [doi:10.25345/C5CR5NQ2N] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: COVID-19 ; Proteomics ; lung injury ; SARS-CoV-2 ; data independent acquisition mass spectrometry ; proximal extension assay ; longitudinal

Contact

Principal Investigators:
(in alphabetical order) 		Shreya Madisetty Kanth, National Institutes of Health, USA
Submitting User: CCMD

Publications

Kanth SM, Huapaya JA, Gairhe S, Wang H, Tian X, Demirkale CY, Hou C, Ma J, Kuhns DB, Fink DL, Malayeri A, Turkbey E, Harmon SA, Chen MY, Regenold D, Lynch NF, Ramelli S, Li W, Krack J, Kuruppu J, Lionakis MS, Strich JR, Davey R, Childs R, Chertow DS, Kovacs JA, Parizi PT, Suffredini AF, COVID-ARC Study Group.
Longitudinal analysis of the lung proteome reveals persistent repair months after mild to moderate COVID-19.
Cell Rep Med. Epub 2024 Jun 27.

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