We describe design, rapid assembly, and characterization of Sc2.0 chromosome VI, synVI. A mitochondrial defect in the synVI strain mapped to synonymous coding changes within PRE4 (YFR055W), encoding an essential proteasome subunit; Sc2.0 coding changes reduced Pre4 protein accumulation by half. Completing Sc2.0 specifies consolidation of sixteen synthetic chromosomes into a single strain. We investigated phenotypic, transcriptional, and proteome-wide consequences of Sc2.0 chromosome consolidation in poly-synthetic strains.
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: synthetic chromosome
Principal Investigators: (in alphabetical order) |
Jef D. Boeke, New York University Langone School of Medicine, USA |
Submitting User: | shruti04 |
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Owner | Reanalyses | |
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