MassIVE MSV000080584

Partial Public

Synthesis, debugging and effects of synthetic chromosome consolidation: synVI and beyond

Description

We describe design, rapid assembly, and characterization of Sc2.0 chromosome VI, synVI. A mitochondrial defect in the synVI strain mapped to synonymous coding changes within PRE4 (YFR055W), encoding an essential proteasome subunit; Sc2.0 coding changes reduced Pre4 protein accumulation by half. Completing Sc2.0 specifies consolidation of sixteen synthetic chromosomes into a single strain. We investigated phenotypic, transcriptional, and proteome-wide consequences of Sc2.0 chromosome consolidation in poly-synthetic strains. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: synthetic chromosome

Contact

Principal Investigators:
(in alphabetical order)
Jef D. Boeke, New York University Langone School of Medicine, USA
Submitting User: shruti04
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Identification Results
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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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