MassIVE MSV000095353

Partial Public PXD053990

Proteomic analysis of the cornified layer of the epidermis on the soles of mice carrying a keratinocyte-specific deletion of Autophagy-related gene 7 (Atg7)

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Description

Keratinocytes of the epidermis undergo massive remodeling of intracellular structures to enter the stratum corneum. This work investigates the degree to which autophagy participates in the remodeling by comparing corneocytes from the sole (foot pads) of wild type (WT, n=5, samples names NK70-74) to those from mice (EKO, n=6, samples names NK75-80) with epidermal keratinocyte-specific deletion of Autophagy-related gene 7 (Atg7). Sample NK81 is a blank. The cornified layer of the epidermis (stratum corneum) was sampled by tape-stripping from the soles of hind limbs and subjected to label-free proteomics as previously described (Karim et al. PLoS One 2023;18:e0283619). Among more than 170 proteins detected, 10 proteins were significantly increased in abundance when autophagy was suppressed. The autophagy-dependent alterations in the protein composition of corneocytes are less pronounced than those in hard cornified skin appendages. Since the proteome of the stratum corneum depends partly on autophagy in keratinocytes, it may be used for the detection of autophagy-dependent aberration of keratinocyte differentiation. [doi:10.25345/C5M03Z83V] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: autophagy ; epidermis

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Principal Investigators:
(in alphabetical order) 		Leopold Eckhar, Department of Dermatology, Medical University of Vienna, Vienna, Austria
Submitting User: brettsp1
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