MassIVE MSV000094586

Complete Public PXD051618

Label-free quantitative data dependent (DDA) nano-LC-MS/MS proteomic profiling of mouse lymph harvested from mesenteric and cervical anatomical districts

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Description

To fully quantify differences in the lymph composition and associated dendritic cells antigenic load, from different anatomical districts and in physiological and pathological conditions, we collected the afferent lymph draining to the cervical and mesenteric lymph nodes in healthy mice. Most of the proteome present in the mesenteric afferent lymph, showed a profile of proteins involved in different metabolic pathways associated with lipoproteintransport, and lipid metabolism, such as adipocyte-type fatty acid binding protein, Apolipoproteins A, B, C and E, and phospholipid transfer proteins, consistent with the known role of the mesenteric lymph in chylomicron transport. Network analysis on the mesenteric afferent lymph unique/enriched proteome highlighted pathways associated with lipase and hydrolase activity, lipoproteins remodeling, fat digestion and absorption, triglyceride catabolism and gut-associated immune cells and cytokines responses. Among the proteome shared across tissue a brain-specific or highly enriched proteome including glia maturation factor, nerve growth factor, mesencephalic astrocyte-derived neurotrophic factor, alpha-crystallin, brain-specific isoform of glycogen phosphorylase, and proteins associated with voltage-dependent channels, were uniquely observed in the lymph harvested from the afferent lymphatics entering the deep cervical nodes. Network analysis on the afferent cervical unique/enriched proteome highlighted pathways associated with neurotransmitter release cycle, synaptic transmission, neuronal development, mitochondrial activity, and an overall CNS proteome. [doi:10.25345/C5PN8XS2R] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: mouse ; mesenteric and cervical lymph ; label free quantitative proteomics profiling on QEHF mass spectrometer

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Principal Investigators:
(in alphabetical order) 		Cristina C Clement, Weill Cornell Medicine College, United States
Laura Santambrogio, Weill Cornell Medicine College, United States
Submitting User: oriongalaxy
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