State-of-the art photoactivation strategies in chemical biology provide spatiotemporal control and visualization of biological processes. We describe the development of targeted aryl azide activation via deep red light photoredox catalysis and its use in photocatalyzed proximity labeling. We demonstrate that aryl azides are converted to triplet nitrenes via a novel redox-centric mechanism and show that its spatially localized-formation requires both red light and a photocatalyst-targeting modality. This technology was applied in different colon cancer cell systems for targeted protein environment labeling of epithelial cell adhesion molecule (EpCAM). We identified a small subset of proteins with previously known and unknown association to EpCAM, including CDH3, a clinically relevant protein that shares high tumor selective expression with EpCAM.
[doi:10.25345/C5X05XF0S]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: proximity labeling
Principal Investigators: (in alphabetical order) |
Olugbeminiyi Fadeyi, Merck, USA Rob Oslund, Merck, USA Tomislav Rovis, Columbia University, USA |
Submitting User: | ryukeu |
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Owner | Reanalyses | |
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