Adult oligodendrocyte progenitors (aOPCs) share with their neonatal counterpart (nOPC) the ability to give rise to myelinating oligodendrocytes, but they also display unique functional features. This study addresses the molecular mechanisms underlying the intrinsic differences between these two populations. Using RNA-sequencing and unbiased histone proteomics analysis, we define the unique transcriptome and histone marks of aOPCs. We used an unbiased histone proteomic approach to ask whether differences in post-translational modifications of nucleosomal histones may underlie the distinct transcriptome of nOPCs and aOPCs. Using Pdgfra-H2BEGFP reporter mice, we sorted for the nuclei of nOPCs and aOPCs, extracted their histones and then conducted a proteomic analysis, which identified several histone modifications of lysine residues in the tails of histones H3 and H4.
[doi:10.25345/C57P8TQ1F]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: histone modifications ; oligodendrocyte progenitors
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Patrizia Casaccia, The Graduate Center, City University of New York, USA |
Submitting User: | Trixi |
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