MassIVE MSV000079947

Imported Reanalysis Dataset Public PXD001326

Proteome profiling of breast cancer biopsies reveals a wound healing signature of cancer-associated fibroblasts - bulk tumor tissue

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Description

Breast cancer is still the most common type of cancer in women; an important role in carcinogenesis is actually attributed to cancer-associated fibroblasts. In this study we investigated whether it is possible to assess the functional state of cancer-associated fibroblasts through tumor tissue proteome profiling. Tissue proteomics was performed on tumor-central, tumor-near and tumor-distant biopsy sections from breast adenocarcinoma patients, which allowed us to identify 2074 proteins. Data were interpreted referring to reference proteome profiles generated from primary human mammary fibroblasts comprising 4095 proteins. These cells were analyzed in quiescent cell state, as well as after in vitro treatment with TGFβ or IL-1β, stimulating wound healing or inflammatory processes, respectively. Representative for cancer cells, we investigated the mammary carcinoma cell line ZR-75-1, identifying 5212 proteins. Comparison of tissue proteomics data with all those reference profiles revealed predominance of cancer cell-derived proteins within the tumor and fibroblast-derived proteins in the tumor-distant tissue sections. Remarkably, proteins characteristic for acute inflammation were hardly identified in the tissue samples. In contrast, several proteins found by us to be induced by TGFβ in mammary fibroblasts, including fibulin-5, SLC2A1 and MUC18, were positively identified in all tissue samples, with relatively higher abundance in tumor neighboring tissue sections. These findings indicate a predominance of cancer-associated fibroblasts with wound healing activities localized around tumors. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Breast cancer ; cancer-associated fibroblasts ; comparative proteome profiling ; label-free quantification ; primary human cells ; tissue proteomics.

Contact

Principal Investigators:
(in alphabetical order) 		Dr Christopher Gerner
Submitting User: ccms

Publications

Groessl M, Slany A, Bileck A, Gloessmann K, Kreutz D, Jaeger W, Pfeiler G, Gerner C.
Proteome profiling of breast cancer biopsies reveals a wound healing signature of cancer-associated fibroblasts.
J. Proteome Res. 2014 Nov 7;13(11):4773-82. Epub 2014 Oct 3.

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