MassIVE MSV000079151

Partial Public

GNPS LCMS data from oral isolates during in vitro metabolism of sugar and lactate

Description

Several oral bacterial species were grown anaerobically and fed either glucose or lactate in SHI medium (for method see: Edlund et al., 2013, Edlund et al, 2015 - see http://www.microbiomejournal.com/content/pdf/2049-2618-1-25.pdf & http://www.nature.com/ismej/journal/vaop/ncurrent/full/ismej201572a.html). Biofilm samples for LCMS were collected at different time points from growth wells. After growth was established in SHI medium, biofilms were washed with minimal medium and supplemented with either glucose or lactate (Veillonella species) (see Edlund et al., 2013, Edlund et al., 2015). Samples were collected both after the initial biofilm establishment in SHI medium and after the addition of the supplemented cdm medium. According to gnps network analyses and data sorting exercises: 1) ~10% of this data is reproducible; 2) several of the present masses can also be detected both in SHI and cdm medium. mzXML files for media is not uploaded here (i.e. no media subtraction can be employed using these files only). [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: oral bacteria

Contact

Principal Investigators:
(in alphabetical order)
Edlund, McLean, Dorrestein
Submitting User: aedlund
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Experimental Design
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Identification Results
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Quantification Results
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GNPS content goes here (MSV000079151 [task=9475e963e18b4369b6c38f9d74c23463])
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.