MassIVE MSV000088001

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Lung cancer plasma glycoproteome correlation with plasma DSC thermograms

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Description

Blood plasma from patients diagnosed with different subtypes of lung cancer or subjects with benign nodules was analyzed by using isobaric labeling and correlating to plasma thermograms. Plasma samples were digested using a FASP approach, labeled with TMT11plex reagents, admixed, deglycosylated using NEB Deglycoslylation Mix II in a non-denaturing approach. Deglycosylated peptides were eluted and analyzed by 1DLC-(low pH RP C-18)-MS. Collected data were analyzed by PD 2.1 using SequestHT and Mascot considering N-linked de-glycosylation of plasma proteins with Asn>Asp and Gln>Glu conversions along with standard modifications (TMT, Met(ox), Cys(CAM)) using an Orbitrap ELITE mass spectrometer. TMT reporter ion values were normalized to pooled internal standard TMT131 label and median glycopeptide abundance values of selected proteins were compared between control subjects and LC patients. [doi:10.25345/C5PG3P] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: lung cancer ; differential scanning calorimetry ; glycoproteomics

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Principal Investigators:
(in alphabetical order) 		Michael L. Merchant, PhD, University of Louisville, USA
Submitting User: mmerchant
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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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