MassIVE MSV000088414

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Mass Spectrometry Imaging of N-Linked Glycans in FFPE Human Prostate by Infrared Matrix-Assisted Laser Desorption Electrospray Ionization

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Description

N-linked glycans are structurally diverse polysaccharides that represent significant biological relevance due to their involvement in disease progression and cancer. Due to their complex nature, N-linked glycans pose many analytical challenges requiring the continued development of analytical technologies. Infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) is a hybrid ionization technique commonly used for mass spectrometry imaging (MSI) applications. Previous work demonstrated IR-MALDESI to significantly preserve sialic acid containing N-linked glycans that otherwise require chemical derivatization prior to detection. Here we demonstrate the first analysis of N-linked glycans in situ by IR-MALDESI MSI. Formalin-fixed paraffin-embedded (FFPE) human prostate tissue was analyzed in negative ionization mode after tissue washing, antigen retrieval, and pneumatic application of PNGase F for enzymatic digestion of N-linked glycans. 53 N-linked glycans were confidently identified in the prostate sample where more than 60% contained sialic acid residues. This work demonstrates the first steps in N-linked glycan imaging of biological tissues by IR-MALDESI MSI. [doi:10.25345/C56Z9F] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: IR-MALDESI ; N-Linked Glycans ; Mass Spectrometry Imaging ; Prostate Cancer

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Principal Investigators:
(in alphabetical order) 		David C. Muddiman, North Carolina State University, United States of America
Submitting User: clgunth2
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