MassIVE MSV000084163

Partial Public

GNPS - Small RNA directs symbiosis, virulence, and natural products biosynthesis in entomopathogenic bacteria

Description

Rapid modulation of gene expression is a key feature for the success of bacteria, particularly for those that rapidly have to adapt to different niches. The lifecycles of Photorhabdus and Xenorhabdus involve a mutualistic association with nematodes as well as an entomopathogenic phase, both of which rely on the production of numerous specialized metabolites (SMs). Several regulators have been previously implicated in the regulation of SM production in these genera. However, the molecular mechanisms underlying the regulatory control of SM production, and especially the role of small regulatory RNAs, are unknown. Here we show that the Hfq-dependent small RNA, ArcZ, is required for SM production. We discovered that ArcZ directly base-pairs with the mRNA encoding HexA, a known repressor of SM production. We further demonstrate that the ArcZ regulon is not restricted to SM production, but rather modulates up to ~15% of the transcriptional output in both Photorhabdus and Xenorhabdus. Together, our study shows that small RNAs are key for SM production in these species, provides new targets for biosynthetic pathway manipulations, and offers a new tool for the (over-)production, isolation and identification of unknown natural products. [doi:10.25345/C5TS99] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: ArcZ, Hfq, HexA, specialized metabolites, sRNA, regulation, Xenorhabdus, Photorhabdus

Contact

Principal Investigators:
(in alphabetical order)
Prof. Helge B. Bode, Goethe Universit?t, Germany
Submitting User: neubacni
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GNPS content goes here (MSV000084163 [task=a263d9c9506247f08cf46b755fd365d7])
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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
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