MassIVE MSV000086332

Complete Public PXD022082

Novel drought adaptation strategies identified by comparing organelle- and cytoplasmic-enriched protein profiles of two Sorghum bicolor genotypes (RTx430 and BTx642) with differing pre-flowering drought tolerance

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Description

Proteomics of organelle and cytoplasmic enriched samples from Sorghum bicolor RTx430 (pre-flowering drought tolerant) and RTx642 (pre-flowering drought sensitive) before and during drought. 3 biological replicates, except BTx642 drought samples, which had 2 biological replicates due to limited sample. Identified multiple protein-level responses in both genotypes, demonstrated that these responses are consistent with the literature, as well as identified multiple RTx430-specific changes that suggest novel drought-tolerance strategies unique to RTx430. Sorghum leaves (both genotypes, +/- drought) were separated into cytosolic and organelle fractions, then digested with trypsin, and analyzed by LC-MS/MS. Data was searched using MaxQuant's Andromeda and a UniProt database. Data was then evaluated for missingness, normalized, and missing values imputed using Bioconductor R package DEP (Zhang et al. 2018). [doi:10.25345/C5RR32] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: drought ; pre-flowering ; rubisco ; abscisic acid ; Flowering Locus C

Contact

Principal Investigators:
(in alphabetical order) 		Pubudu P. Handakumbara, Pacific Northwest National Laboratory, USA
Submitting User: alchemistmatt

Publications

Ogden AJ, Abdali S, Engbrecht KM, Zhou M, Handakumbura PP.
Distinct Preflowering Drought Tolerance Strategies of Sorghum bicolor Genotype RTx430 Revealed by Subcellular Protein Profiling.
Int J Mol Sci. Epub 2020 Dec 19.

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