In this study we have used an unbiased approach to determine the impact of the ettA deletion (delta ettA) on the physiology of E. coli. The gene deletion creates a hypersensitivity to salt when the bacteria are grown on carbon sources metabolized by the TCA cycle. This phenotype is due to the reduced translation of several genes of the TCA cycle and of genes involved in stress responses. In this work we have systematically compared three strains MG1655 WT, delta ettA and the delta ettA complemented with an exogenous chromosomal copy of ettA (CettA). Label-Free mass spectrometry studies performed on light (S15) and heavy (S150) fractions of proteins extracts from cultures grown in MMAA-NaCl medium, identified several protein expression changes in the delta ettA strain compared to the WT or the CettA strains.
[doi:10.25345/C5474727V]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: ABC-F protein family ; quantification ; label-free ; mass spectrometry
Principal Investigators: (in alphabetical order) |
Gregory Boel, UMR 8261, IBPC, CNRS, France |
Submitting User: | marion_H |
Number of Files: | |
Total Size: | |
Spectra: | |
Subscribers: | |
Owner | Reanalyses | |
---|---|---|
Experimental Design | ||
Conditions:
|
||
Biological Replicates:
|
||
Technical Replicates:
|
||
Identification Results | ||
Proteins (Human, Remapped):
|
||
Proteins (Reported):
|
||
Peptides:
|
||
Variant Peptides:
|
||
PSMs:
|
||
Quantification Results | ||
Differential Proteins:
|
||
Quantified Proteins:
|
||
Browse Dataset Files | |
Browse Quantification Results | |
FTP Download Link (click to copy):
|