MassIVE MSV000086539

Complete Public PXD022828

The neomycin resistance cassette in the targeted allele of Shank3B knock-out mice has potential off-target effects to produce an unusual Shank3 isoform

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Description

Variants of the SH3 and multiple ankyrin repeat domains 3 (SHANK3), which encodes postsynaptic scaffolds, are associated with brain disorders. The targeted alleles in a few Shank3 knock-out (KO) lines contain a neomycin resistance (Neo) cassette, which may perturb the normal expression of neighboring genes; however, this has not been investigated in detail. We previously reported an unexpected increase in the mRNA expression of Shank3 exons 1~12 in the brains of Shank3B KO mice generated by replacing Shank3 exons 13~16 with the Neo cassette. In this study, we confirmed that the increased Shank3 mRNA in Shank3B KO brains produced an unusual ~60 kDa Shank3 isoform (Shank3-N), which did not properly localize to the synaptic compartment. [doi:10.25345/C5149M] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: shank3 ; neomycin

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Principal Investigators:
(in alphabetical order) 		Jin Young Kim, Korea Basic Science Institute, Rep. of Korea
Submitting User: tilldawn
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