The aim of the study was to investigate the effects of ionizing radiation on CT-2A cells at the protein level, proteomics analyses were performed on CT-2A cells irradiated with 5 Gy. To investigate the effect of glioma-secreted extracellular vesicles (EVs) on bone-marrow-derived macrophages (BMDM) at the protein level, BMDM were exposed to EVs for 48 hours.
Multiplexed mass spectrometry-based proteomics was performed using TMTpro barcoding reagents and the SPS-MS3 method on an Orbitrap Lumos mass spectrometer.
Samples were labeled as follows:
CT-2A CTRL samples: CT2A_Cells_CTRL_1 (126), CT2A_Cells_CTRL_2 (127n)
CT-2A IR samples: CT2A_Cells_IR_1 (127c), CT2A_Cells_IR_2 (128n)
BMDM samples: Macrophage_CTRL_1 (128c), Macrophage_CTRL_2 (129n)
BMDM+EV samples: Macrophage_Plus_EV_1 (130c), Macrophage_Plus_EV_2 (131n)
BMDM+irEV samples: Macrophage_Plus_irEV_1 (131c), Macrophage_Plus_irEV_2 (132n)
[doi:10.25345/C5JH3DD1M]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Extracellular Vesicles, GBM, Radiation, PD-L1, Immunosuppression, Macrophages, TMTpro, Orbitrap Lumos, SPS-MS3
Principal Investigators: (in alphabetical order) |
Bakhos Tannous, Massachusetts General Hospital and Harvard Medical School (Present address: Early Oncology R&D, ICC, AstraZeneca, Waltham, MA02451, USA), USA Koen Breyne, Massachusetts General Hospital and Harvard Medical School, USA Wilhelm Haas, Massachusetts General Hospital and Harvard Medical School, United States |
Submitting User: | whaas |
Number of Files: | |
Total Size: | |
Spectra: | |
Subscribers: | |
Owner | Reanalyses | |
---|---|---|
Experimental Design | ||
Conditions:
|
||
Biological Replicates:
|
||
Technical Replicates:
|
||
Identification Results | ||
Proteins (Human, Remapped):
|
||
Proteins (Reported):
|
||
Peptides:
|
||
Variant Peptides:
|
||
PSMs:
|
||
Quantification Results | ||
Differential Proteins:
|
||
Quantified Proteins:
|
||
Browse Dataset Files | |
FTP Download Link (click to copy):
|