MassIVE MSV000094439

Complete Public PXD051100

Coupland et al., Cryo-EM of synaptic vesicles reveals loading-induced V-ATPase dissociation

Description

This dataset consists of 4 raw mass spectrometry files and associated peak lists and results files, acquired on a Bruker timsTOF Pro 2 mass spectrometer operated in Data-Dependent Acquisition mode. Synaptic vesicle and cleared rat brain lysate samples were prepared and separated in SDS-PAGE gel, then processed for in-gel protease digestion by Claire Coupland. Mass spectrometric acquisition and database searches were performed by Network Biology Collaborative Centre at the Lunenfeld-Tanenbaum Research Institute. Mass spec results were analyzed by Ji-Young Youn and John Rubinstein. The files are associated with a manuscript by Coupland et al. that determines the structure of synaptic vesicle V-ATPases and their associated proteins using Cryo-EM structural analysis. Mass spectrometry analysis is used to determine the protein components in purified synaptic vesicles. John Rubinstein is the corresponding author for the manuscript and should be contacted for questions regarding this dataset (john.rubinstein@utoronto.ca). [doi:10.25345/C5PG1J030] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Cryo electron microscopy, v-ATPase, synaptic vesicles

Contact

Principal Investigators:
(in alphabetical order)
John Rubinstein, Molecular Medicine Program, The Hospital for Sick Children; Department of Biochemistry and Department of Medical Biophysics, The University of Toronto, Canada
Submitting User: Younlab_Toronto
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Experimental Design
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Identification Results
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Quantification Results
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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.