MassIVE MSV000092786

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Genomics and metabolomics-based assessment of the biosynthetic potential of the sponge-associated microorganism Streptomyces cacaoi strain R2A-843A from the Philippines

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Description

The biosynthetic machinery of the sponge-associated Streptomyces cacaoi strain R2A-843A was assessed using a combined genomics and metabolomics approach. Whole genome sequencing and molecular networking showed the high biosynthetic potential of this actinomycete. A significant proportion of the genome is dedicated to secondary metabolite production, with biosynthetic gene clusters for nonribosomal peptides, polyketides and terpenes being the most represented. Seven cyclic pentapeptides, including a putative new analogue, and a glycosylated lanthipeptide were identified using HRMS and untargeted MSMS analysis. Chemistry-guided purification confirmed the production of the peptides BE-18257A (1) and BE-18257B (2). The production of 1 and 2 and the growth of the microorganism were monitored for eight days. Compound 2 was produced at higher concentration, starting at 48 h post-incubation. [doi:10.25345/C5WH2DR29] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Actinobacteria ; BE-18257 ; genome ; metabolome ; pentaminomycins ; secondary metabolites ; Streptomyces

Contact

Principal Investigators:
(in alphabetical order) 		Lilibeth A. Salvador-Reyes, The Marine Science Institute, University of the Philippines Diliman, Philippines
Submitting User: zlmalto
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