MassIVE MSV000085028

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Identification of PIM1 substrates reveals a role for NDRG1 in prostate cancer cellular migration and invasion

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Description

PIM1 is an oncogenic serine/threonine kinase that promotes and maintains prostate tumorigenesis. To more fully understand the mechanism by which PIM1 promotes oncogenesis, we performed a chemical genetic screen to identify direct PIM1 substrates in prostate cancer cells. To identify PIM1 substrates and their phosphorylation sites in LNCaP cells, we coupled a chemical genetic screen with a peptide capture, mass spectrometry (MS)-based approach. We mutated the PIM1 gatekeeper residue in the ATP binding site to accept a bulky ATP analog. By using an ATP analog labeled with a thiol group on the gamma-phosphate, we were able specifically label PIM1 substrates even in the presence of other cellular kinases. [doi:10.25345/C5VH73] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: PIM1 substrates ; phosphorylation ; analog-sensitive kinase-substrate detection method

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Principal Investigators:
(in alphabetical order) 		Beatrix Ueberheide, NYU School of Medicine, USA
Submitting User: Trixi
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