We developed a set of algorithms for label-free quantification, termed MaxLFQ, embedded into MaxQuant. This contains two datasets to benchmark MaxLFQ: The proteome benchmark dataset consists of of HeLa and E. coli lysates mixed at defined ratios. The dynamic range benchmark dataset consists of UPS1/UPS2 standards (Sigma) spiked into E. coli lysates and quantified against each other.
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: label-free quantification ; MaxQuant ; MaxLFQ ; benchmark datasets ; Controlled mixture ; MassIVE.quant reviewed - Platinum
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Submitting User: | ccms |
Cox J, Hein MY, Luber CA, Paron I, Nagaraj N, Mann M.
Accurate proteome-wide label-free quantification by delayed normalization and maximal peptide ratio extraction, termed MaxLFQ.
Mol. Cell Proteomics. 2014 Sep;13(9):2513-26. Epub 2014 Jun 17.
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