22 week old female Tfamfl/fl (aka WT), BEST1Cre;Tfamfl/fl, BEST1Cre;Tfamfl/fl;AN/+ mice were fasted for 6 hrs (8am-2pm), then were retro-orbital injected with 13C6-glucose (Cambridge Isotopes Laboratories): 250mg/kg in 100-135ul saline and 0.2 um sterile filtered. Tissues were processed 30min after injection. A cryolysis method was used to extract RPE metabolites. NR metabolites were extracted as usual.
[doi:10.25345/C5QZ22V0G]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: metabolism ; fasting
Principal Investigators: (in alphabetical order) |
Jianhai Du, West Virginia University, United States |
Submitting User: | jianhaidulab |
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