Protein translational control is a highly regulated step in the gene expression program during development and tools to quantify protein synthesis rates in a developing fetus are lacking. Here, we developed a novel in utero approach to quantify tissue-specific translational kinetics of the nascent proteome during mouse fetal development. The translational kinetic profiles of developing organs displayed differentially expressed protein pathways and synthesis rates which correlated with known physiological changes during mouse development.
[doi:10.25345/C55R79]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: protein turnover ; pSILAC ; pulse-SILAC ; pulse chase ; development ; in utero
Principal Investigators: (in alphabetical order) |
Benjamin A. Garcia, Department of Biochemistry and Biophysics, University of Pennsylvania, N/A |
Submitting User: | baeza |
Baeza J, Coons BE, Lin Z, Riley J, Mendoza M, Peranteau WH, Garcia BA.
In utero pulse injection of isotopic amino acids quantifies protein turnover rates during murine fetal development.
Cell Rep Methods. 2024 Feb 26;4(2):100713.
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