The novel coronavirus SARS-CoV-2 is responsible for the ongoing COVID-19 pandemic and has caused a major health and economic burden worldwide. Understanding how SARS-CoV-2 viral proteins behave in host cells can reveal underlying mechanisms of pathogenesis and assist in development of antiviral therapies. Here the cellular impact of expressing SARS-CoV-2 viral proteins was studied by global proteomic analysis and proximity biotinylation (BioID) was used to map the SARS-CoV-2 virus-host interactome in human lung cancer derived cells. Functional enrichment analyses revealed previously reported and unreported cellular pathways that are associated with SARS-CoV-2 proteins. We have also established a website to host the proteomic data to allow for public access and continued analysis of host-viral protein associations and whole-cell proteomes of cells expressing the viral-BioID fusion proteins. Furthermore, we identified 154 high-confidence interactions by comparing this study with previous reports, providing a strong foundation for future follow-up studies. Finally, we cross-referenced candidate interactors with the CLUE drug library to identify potential therapeutics for drug-repurposing efforts. Collectively, these studies provide a valuable resource to uncover novel SARS-CoV-2 biology and inform development of antivirals.
[doi:10.25345/C5684N]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: SARS-Cov2 ; BioID ; protein-protein interaction ; Shiny application
Principal Investigators: (in alphabetical order) |
Alex Rosa Campos, Sanford Burnham Prebys Medical Discovery Institute, United States |
Submitting User: | alexcampos |
May DG, Martin-Sancho L, Anschau V, Liu S, Chrisopulos RJ, Scott KL, Halfmann CT, Díaz Peña R, Pratt D, Campos AR, Roux KJ.
A BioID-Derived Proximity Interactome for SARS-CoV-2 Proteins.
Viruses. Epub 2022 Mar 15.
Number of Files: | |
Total Size: | |
Spectra: | |
Subscribers: | |
Owner | Reanalyses | |
---|---|---|
Experimental Design | ||
Conditions:
|
||
Biological Replicates:
|
||
Technical Replicates:
|
||
Identification Results | ||
Proteins (Human, Remapped):
|
||
Proteins (Reported):
|
||
Peptides:
|
||
Variant Peptides:
|
||
PSMs:
|
||
Quantification Results | ||
Differential Proteins:
|
||
Quantified Proteins:
|
||
Browse Dataset Files | |
FTP Download Link (click to copy):
|