MassIVE MSV000081954

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Shenmai iTRAQ proteome

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Description

To explore the mechanism underlying cardioprotective effects of SM, iTRAQ-based proteomic approach was applied to analyze protein of myocardium in rats with myocardial ischemic injury. Myocardial tissue of Shenmai-treated mice with left coronary artery ligation were harvest after 7 days treatment. Tissue samples were homogenized with lysis buffer by needle sonication. The solubilized samples were centrifuged at 16,000 � g for 10 min at 4 �C and the total protein in the supernatant was quantified using BCA assay. The relative quantification was calculated based on the ratio of the reporter ions intensities at m/z 117.1, 118.1, 119.1, and 121.1 corresponding to the myocardial ischemia condition as well as treated with RG, OP, and SM, over the reporter ion intensity at m/z 116.1 corresponding to sham-operated condition. The peak lists were submitted for database sequence searches using ProteinPilot software (version 3.0, Applied Biosystems). The following parameters were set in the searching: trypsin as enzyme, fixed modification of carbamidomethyl labeled cysteine, iTRAQ as sample type, no special factors, biological modification, thorough identification search. Other parameters, such as tryptic cleavage specificity, precursor ion mass accuracy, and fragment ion mass accuracy were built-in functions of ProteinPilot software, and the Paragon method was adopted. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Shenmai Formula, iTRAQ proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Yi Wang, Zhejiang University, China
Submitting User: zhaoyujoy
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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
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