MassIVE MSV000086249

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Analysis of the Yarrowia lipolytica proteome reveals subtle variations in expression levels between lipogenic and non-lipogenic conditions

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Description

Y. lipolytica cells were grown in rich-medium and (nutrient limited) minimal-medium, showing accumulations of neutral lipids at 10% and 30% of dry cell weight respectively. To study the mechanisms driving this, we sampled these cultures in paired replicates during both log and stationary phases of the cell growth, and performed 1D LC-MS/MS quantitation analysis on their tryptic digests. Sample assignments were as follows: (RME1, RME2) log phase rich; (RMG3, RMG4) stationary phase rich; (RME10, RME11) log phase minimal; (RME7, RME8) stationary phase minimal. Label free quantitation yielded a log2 expression matrix of over 1800 proteins. Differential analysis showed that while the hypothesized driver of TAG storage- ATP citrate lyase- remained stable, there was up-regulation of proteins involved in the TCA cycle. [doi:10.25345/C57784] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: biofuels ; lipids ; label-free quantitation

Contact

Principal Investigators:
(in alphabetical order) 		David Levin, University of Manitoba, Canada
Submitting User: vspicer1

Publications

Sestric R, Spicer V, V Krokhin O, Sparling R, B Levin D.
Analysis of the Yarrowia lipolytica proteome reveals subtle variations in expression levels between lipogenic and non-lipogenic conditions.
FEMS Yeast Res.

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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
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