Y. lipolytica cells were grown in rich-medium and (nutrient limited) minimal-medium, showing accumulations of neutral lipids at 10% and 30% of dry cell weight respectively. To study the mechanisms driving this, we sampled these cultures in paired replicates during both log and stationary phases of the cell growth, and performed 1D LC-MS/MS quantitation analysis on their tryptic digests.
Sample assignments were as follows: (RME1, RME2) log phase rich; (RMG3, RMG4) stationary phase rich; (RME10, RME11) log phase minimal; (RME7, RME8) stationary phase minimal.
Label free quantitation yielded a log2 expression matrix of over 1800 proteins.
Differential analysis showed that while the hypothesized driver of TAG
storage- ATP citrate lyase- remained stable, there was up-regulation of proteins
involved in the TCA cycle.
[doi:10.25345/C57784]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: biofuels ; lipids ; label-free quantitation
Principal Investigators: (in alphabetical order) |
David Levin, University of Manitoba, Canada |
Submitting User: | vspicer1 |
Sestric R, Spicer V, V Krokhin O, Sparling R, B Levin D.
Analysis of the Yarrowia lipolytica proteome reveals subtle variations in expression levels between lipogenic and non-lipogenic conditions.
FEMS Yeast Res.
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