MassIVE MSV000081430

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Profiling B-Type Natriuretic Peptide Cleavage Peptidoforms in Human Plasma by Capillary Electrophoresis with Electrospray Ionization Mass Spectrometry.

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Description

: B-type Natriuretic Peptide (BNP) is a biologically active circulating hormone. Plasma concentrations of BNP are routinely used in the diagnosis of heart failure and the intravenous infusion of recombinant BNP can be used for heart failure treatment. We sought to develop a technique capable of profiling these catabolic processes in plasma. We used a neutral-coated Capillary Electrophoresis-Electrospray Ionization (CESI) separation system coupled with high-resolution mass spectrometry to profile the proteolysis of exogenous recombinant BNP1-32 in plasma. Our method utilizes electrokinetic injection of minimally processed plasma samples to simultaneously monitor the dynamic generation and breakdown of at least five BNP peptidoforms in plasma. By integrating multisegment injection, our method can produce a multi-point BNP proteolytic profile for one sample within an hour. We envision applying this method to assess the potential relation between plasma-based BNP proteolysis and heart failure, as well as a means of monitoring BNP bioavailability after therapeutic infusion. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Capillary Electrophoresis, Mass Spectrometry, CESI 8000, Peptidoforms, Brain Natriuretic Peptide, Multisegment Injec-tion, Neutral Capillary, proteolysis profile.

Contact

Principal Investigators:
(in alphabetical order) 		Jennifer Van Eyk, Cedars Sinai Medical Center, United States
Submitting User: Gloria1115
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