Male rhesus macaques (Macaca mulatta), cleared of previous infection with P. cynomolgi B strain via treatment with the anti-malarial drugs artemether, chloroquine, and primaquine, approximately five years of age, were inoculated intravenously with salivary gland sporozoites produced and isolated at the Centers for Disease Control and Prevention from multiple Anopheles species (An. dirus, An. gambiae, and An. stephensi) and then profiled for clinical, hematological, parasitological, immunological, functional genomic, lipidomic, proteomic, and metabolomic measurements. The experiment was conducted for 51 days, and pre- and post-51 day periods to prepare subjects and administer post-experiment curative treatments respectively. The anti-malarial drug artemether was subcuratively administered to subjects at the initial peak of infection, if subjects were not able to self-resolve their parasitemias. Peak infection was determined clinically for each subject. The anti-malarial drugs primaquine and chloroquine were administered to all subjects at the end of the study for curative treatment of the liver and blood-stage infections, respectively. Capillary blood samples were collected daily for the measurement of CBCs, reticulocytes, and parasitemias. Capillary blood samples were collected every other day to obtain plasma for metabolomic analysis. Venous blood samples were collected at five time points for functional genomic, lipidomic, proteomic, and immunological analyses. Within the MaHPIC, this project is known as 'Experiment 25'. This is the third and final of a series of experiments that includes infection of malaria-naïve subjects (Experiment 23, P. cynomolgi B strain) and homologous challenge (Experiment 24, P. cynomolgi B strain) of individuals from the same cohort. This dataset was produced by: The MaHPIC Consortium, Monica Cabrera-Mora, Jeremy D. DeBarry, Mary R. Galinski, Jay C. Humphrey, Ebru Karpuzoglu, Jessica C. Kissinger, Regina Joice Cordy, Esmeralda VS Meyer, Alberto Moreno, Mustafa V. Nural, Daniel S. Ory, Suman B Pakala, and Xuntian Jiang. The experimental design and protocols for this study were approved by the Emory University Institutional Animal Care and Use Committee (IACUC).
For more information on the MaHPIC, please visit http://www.systemsbiology.emory.edu/. To access other publicly available results from 'E25' and other MaHPIC Experiments, including clinical results (specifics on drugs administered, diet, and veterinary interventions), and other omics, visit http://plasmodb.org/plasmo/mahpic.jsp. This page will be updated as datasets are released to the public."[doi:10.25345/C5504K] [dataset license: CC0 1.0 Universal (CC0 1.0)]
|Principal Investigators:||Mary Galinski, Emory University, USA|
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