MassIVE MSV000092971

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Extracellular matrix proteome remodeling with substrate stiffness

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Description

Extracellular matrix proteomic profiling Mass spectrometry characterization illustrated a reprogrammed proteome in response to matrix stiffness, including a core subset of 84 ECM-specific proteins, including various ECM regulators and tissue-associated ECM-related proteins. Further, we observe remodeling of ECM proteome with softer substrates associated with metabolic/mitochondrial network (178 proteins), while with 40 kPa substrate (92 proteins enriched) resulting in networks associated with enrichment of collagen biosynthesis, integrin cell surface interactions, and extracellular matrix organization networks. [doi:10.25345/C5445HP44] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: extracellular matrix ; ECM ; proteome ; matrix ; biomaterial

Contact

Principal Investigators:
(in alphabetical order) 		David Greening, Baker Heart & Diabetes Institute, Australia
Submitting User: dwgree

Publications

Yang MC, Chin IL, Fang H, Drack A, Nour S, Choi YS, O'Connor AJ, Greening DW, Kalionis B, Heath DE.
Tailored environments for directed mesenchymal stromal cell proliferation and differentiation using decellularized extracellular matrices in conjunction with substrate modulus.
Acta Biomater. Epub 2024 Aug 22.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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