In natural environments, photosynthetic organisms adjust their metabolism to cope with the fluctuating availability of combined nitrogen-sources, a growth limiting factor. For the acclimation, the dynamic degradation/synthesis of tetrapyrrolic pigments as well as of the amino acid arginine is pivotal; however, there was no evidence that these processes could be functionally coupled. Using co-immunopurification and spectral shift assays we found that in the cyanobacterium Synechocystis sp. PCC 6803 the arginine-related ArgD and CphB enzymes form protein complexes with Gun4, an essential factor for chlorophyll biosynthesis. Gun4 binds ArgD with high affinity, and the ArgD-Gun4 complex strongly accumulates in cells supplemented with ornithine, a key intermediate of the arginine pathway. Elevated ornithine levels restricted de novo synthesis of tetrapyrrolic pigments, which arrested the recovery from nitrogen deficiency. Our data reveals a direct cross-talk between tetrapyrrole biosynthesis and arginine metabolism that clarifies the importance of balancing photosynthetic pigment synthesis with nitrogen homeostasis.
[doi:10.25345/C5DB7W09F]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: tetrapyrrole biosynthesis ; Synechocystis ; chlorophyll ; bilins ; Genome-Uncoupled-4 ; arginine metabolism ; nitrogen homeostasis
Principal Investigators: (in alphabetical order) |
Roman Sobotka, 1Laboratory of Photosynthesis, Centre Algatech, Institute of Microbiology, The Czech Academy of Sciences, 37901 Trebon; Faculty of Science, University of South Bohemia, 37005, Ceske Budejovice, Czech Republic |
Submitting User: | konik |
Kiss É, Talbot J, Adams NBP, Opekar S, Moos M, Pilný J, Kvasov T, Schneider E, Koník P, Šimek P, Sobotka R.
Chlorophyll biosynthesis under the control of arginine metabolism.
Cell Rep. 2023 Nov 28;42(11):113265. doi: 10.1016/j.celrep.2023.113265.
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