MassIVE MSV000086049

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Evaluating the performance of 193 nm ultraviolet photodissociaiton (UVPD) for tandem mass tag (TMT) labeled peptides

Description

We evaluated 193 nm UVPD for characterization of TMT labeled peptides (HeLa digest) and N-glycopeptides (alpha-1-acid glycoprotein digest) at different conditions, and compared the results with HCD. Samples were digested with trypsin and labeled with TMT6. Instrument data files were searched with either MS-GF+ (using PNNL's DMS processing pipeline) or with Byonic. [doi:10.25345/C5645N] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: ultraviolet photodissociation ; tandem mass tag ; quantitative proteomics ; glycopeptides

Contact

Principal Investigators:
(in alphabetical order)
Jared B. Shaw, e-MSion, Inc., USA
Submitting User: alchemistmatt

Publications

Mowei Zhou, Ju Yeon Lee, Gun Wook Park, Neha Malhan, Tao Liu, Jared B. Shaw.
Evaluating the Performance of 193 nm Ultraviolet Photodissociation for Tandem Mass Tag Labeled Peptides.
Analytica 2021, 2(4), 140-155; https://doi.org/10.3390/analytica2040014.

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Identification Results
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Quantification Results
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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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