MassIVE MSV000080834

Imported Reanalysis Dataset Public PXD001115

Proteomics of NGF-TrkA signaling dynamics in neuroblastoma cells

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Description

We used quantitative mass spectrometry-based proteomics to unravel global nerve growth factor (NGF)-induced TrkA signaling dynamics at the interactome, phosphoproteome and proteome level. A tetracycline-inducible system for TrkA expression was generated in the human neuroblastoma cell line, SH-SY5Y. TrkA-induced cells were stimulated with NGF for different time points to follow phosphoproteome, interactome and proteome changes on a temporal scale. In a triple SILAC setup (Light: Lys0,Arg0; Medium: Lys4,Arg6; and Heavy: Lys8,Arg10), the samples were stimulated with NGF as indicated. Phosphoproteome: 0, 10, 45 min and 0, 120, 120 min+cycloheximide. Interactome: 0, 5, 10 min. Proteome: 0, 24, 48 h. All experiments were performed as biological replicates. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: NGF ; TrkA ; neuroblastoma ; mass spectrometry ; SILAC ; phosphoproteome ; interactome ; proteome

Contact

Principal Investigators:
(in alphabetical order) 		Jesper V. Olsen, Novo Nordisk Foundation Center for Protein Research Faculty of Health & Medical Sciences University of Copenhagen Blegdamsvej 3B DK-2200 Copenhagen N Denmark, N/A
Submitting User: ccms

Publications

Emdal KB, Pedersen AK, Bekker-Jensen DB, Tsafou KP, Horn H, Lindner S, Schulte JH, Eggert A, Jensen LJ, Francavilla C, Olsen JV.
Temporal proteomics of NGF-TrkA signaling identifies an inhibitory role for the E3 ligase Cbl-b in neuroblastoma cell differentiation.
Sci Signal. 2015 Apr 28;8(374):ra40. Epub 2015 Apr 28.

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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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