MassIVE MSV000083688

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Discrimination of Isomers of Released N- and O-Glycans Using Diagnostic Product Ions in Negative Ion PGC-LC-ESI-MS/MS

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Description

We used porous graphitized carbon-LC-ESI-MS/MS to separate and detect released N- and O-glycan isomers from mammalian model glycoproteins using negative mode resonance activation CID-MS/MS. By interrogating similar fragment spectra from closely related glycan isomers that differ only in arm position and sialyl linkage, product fragment ions for discrimination between these features were discovered. These diagnostic ions were shown to be useful for isomer discrimination using both linear and 3D ion trap mass spectrometers when analyzing complex glycan mixtures from cell lysates. This platform-independent workflow can potentially be extended to automate the characterization and quantitation of other challenging glycan isomers. [doi:10.25345/C5B913] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: diagnostic ions ; Glycomics ; Skyline

Contact

Principal Investigators:
(in alphabetical order) 		Nicolle H. Packer, Macquarie University, Australia
Submitting User: cashwood

Publications

Ashwood C, Lin CH, Thaysen-Andersen M, Packer NH.
Discrimination of Isomers of Released N- and O-Glycans Using Diagnostic Product Ions in Negative Ion PGC-LC-ESI-MS/MS.
J. Am. Soc. Mass Spectrom. 2018 Jun;29(6):1194-1209. Epub 2018 Mar 30.

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