MassIVE MSV000081717

Imported Reanalysis Dataset Public PXD008141

Blood plasma and serum samples of healthy donors fractionated on different chromatography sorbents

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Description

Blood as connective tissue potentially contain evidence of all processes occurring within the organism, at least in trace amounts. Because of their small size, peptides overcome cell membranes and epithelial barriers more freely than proteins. Among the peptides found in blood, there are both fragments of proteins secreted by various tissues and performing their function in plasma, as well as receptor ligands: hormones, cytokines, mediators of the cellular response. In addition, in minor amounts, there are disease peptide markers (for example, oncomarkers) and even foreign peptides related to pathogenic organisms and infection agents. To characterize the normal peptidome LC-MS/MS analysis was carried out of blood serum and plasma samples taken from 20 healthy donors on TripleTOF 5600+ mass-spectrometer. Sample preparation was carried out based on our previously developed method of peptide desorption from the surface of abundant blood plasma proteins followed by standard chromatographic steps. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Healthy donors ; blood plasma and serum ; peptidomics ; mass-spectrometry ; norm

Contact

Principal Investigators:
(in alphabetical order) 		Georgij Pavlovich Arapidi, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, N/A
Submitting User: ccms

Publications

Arapidi G, Osetrova M, Ivanova O, Butenko I, Saveleva T, Pavlovich P, Anikanov N, Ivanov V, Govorun V.
Peptidomics dataset: Blood plasma and serum samples of healthy donors fractionated on a set of chromatography sorbents.
Data Brief. Epub 2018 Apr 10.

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Experimental Design
    Conditions:
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Identification Results
    Proteins (Human, Remapped):
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Quantification Results
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When complete, the converted files will be available in the "ccms_peak" subdirectory of the dataset's FTP space (accessible via the "FTP Download" link to the right).
Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.