MassIVE MSV000080800

Imported Reanalysis Dataset Public PXD003660

Early Signaling Dynamics of EGFR

Description

Despite extensive study of the epidermal growth factor receptor (EGFR) signaling network, the immediate post-translational changes that occur in response to growth factor stimulation remain poorly characterized; as a result, the biological mechanisms underlying signaling initiation remain obscured. To address this deficiency, we have used a mass spectrometry-based approach to measure system-wide phosphorylation changes throughout the network with 10 second resolution in the 80 seconds after stimulation in response to a range of 8 growth factor concentrations. Significant changes were observed on proteins far downstream in the network as early as 10 seconds after stimulation, indicating a system capable of transmitting information quickly. Meanwhile, canonical members of the EGFR signaling network fall into clusters with distinct activation patterns. Shc and PI3K phosphorylation levels increase rapidly, but equilibrate within 20 s, while proteins like Gab1 and SHP2 show slower, sustained increases. Proximity ligation assays reveal that Shc and Gab1 phosphorylation patterns are representative of separate timescales for physical association with the receptor. Inhibition of phosphatases with vanadate reveals site-specific regulatory mechanisms, and also uncovers primed activating components in the network, including Src family kinases, whose inhibition impacts only a subset of proteins within the network. The results presented highlight the complexity of signaling initiation, and provide a window into exploring mechanistic hypotheses about RTK biology. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: EGFR ; Mass spectrometry ; tyrosine phosphorylation ; systems biology

Contact

Principal Investigators:
(in alphabetical order)
Forest White, Massachusetts Institute of Technology, N/A
Submitting User: ccms

Publications

Reddy RJ, Gajadhar AS, Swenson EJ, Rothenberg DA, Curran TG, White FM.
Early signaling dynamics of the epidermal growth factor receptor.
Proc. Natl. Acad. Sci. U.S.A. 2016 Mar 15;113(11):3114-9. Epub 2016 Mar 29.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.