MassIVE MSV000083711

Partial Public

Quantitative proteomics reveals remodeling of protein repertoire across life phases of Daphnia pulex

Description

Microcrustacean Daphnia is becoming a model organism of choice for ecological and developmental genomics. Yet, Daphnia proteomics resources have so far been rudimental. This study presents LC-MS2/SPS-MS3 proteomes of adult females, juveniles, asexually produces embryos and sexually produced diapausing embryos capable of surviving freezing and desiccation. Samples were fractionated using high pH reversed-phase chromatography. 12 farctions were analyzed by LC-MS2/SPS-MS3. The samples are labeled as follows: E1 (128n), E2 (128c), E3 (129n); 3 biological replicates of asexually produced embryos J1 (127n), J2 (127c), J3 (131n); 3 biological replicates of juveniles M1 (129c), M2 (130n), M3 (130c); 3 biological replicates of adult females Eph (126); a single ephippium sample (sexually produced diapausing embryos). [doi:10.25345/C5C34G] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Daphnia pulex, proteome mapping, Orbitrap Lumos, TMT10

Contact

Principal Investigators:
(in alphabetical order)
Lev Yampolski, East Tennessee State University, Biological Sciences, United States
Submitting User: whaas
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Experimental Design
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Identification Results
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Quantification Results
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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
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