MassIVE MSV000087094

Partial Public

Shotgun proteome analysis of homogenate milk samples reveals dynamic changes in milk proteins between colostrum, transitional and mature milk of swine

Description

Milk is an easily digestible source of nutrients and bioactive factors, and its composition reflects the neonate's needs and changes from colostrum to transitional and mature milk. Our objective was to measure milk fat, lactose, total carbohydrate, and protein content in parallel with global proteome of homogenate milk samples to characterize changes across the three phases of swine lactation. Milk samples were collected from multiparous sows on postnatal day 0 (D0: colostrum), 3 (D3: early transitional), 7 (D7: late transitional) and 14 (D14: mature). Liquid chromatography tandem mass spectrometry proteomic analysis of homogenate D0, D3, and D14 milk samples (n=6) identified 772 proteins corresponding to 501 non-redundant protein-coding genes, of which 207 proteins were high confidence (detected in n=3 sows/day). Of the high confidence proteins, 81 were common to all days. Among the proteins that changed between the days was the decrease in multiple apolipoproteins and the milk fat globule membrane protein XDH. There were variable changes in complement factors, where as 14-3-3 proteins (YWHAQ,YWHAE) increased across the days. Our data provide a good characterization of milk proteome changes that likely reflect mammary function as well as the neonate's phase-specific developmental needs. These data, may be useful in developing approaches to enhance the health and welfare of swine. [doi:10.25345/C58Z3S] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Colustrum, proteomics, Sus scrofa, mass spectrometry, label free quantitation

Contact

Principal Investigators:
(in alphabetical order)
Radiah C. Minor, North Carolina A&T State University, United States
Submitting User: uma_aryal
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