A novel 370 kDa translocon comprising Sec61 and five accessory factors acting co-translationally with ribosomes to fold and insert multi-pass membrane proteins was identified, purified and analyzed structurally by single particle cryo-EM and cross-linking mass spectrometry. The purified ribosome-translocon complex was cross-linked with DSS, trypsin digested, and size-exclusion enriched (SEC) cross-link containing fractions were analyzed by sequential HCD/ETD of the same precursor ion. The four LC-MS runs corresponding to SEC fractions eluting between 0.9-1.4 ml are deposited here.
[doi:10.25345/C5D656]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Cross-linking Mass Spectrometry ; ETD ; HCD
Principal Investigators: (in alphabetical order) |
Robert J. Keenan, University of Chicago, USA |
Submitting User: | mtrnka |
McGilvray PT, Anghel SA, Sundaram A, Zhong F, Trnka MJ, Fuller JR, Hu H, Burlingame AL, Keenan RJ.
An ER translocon for multi-pass membrane protein biogenesis.
Elife. Epub 2020 Aug 21.
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