Cooper sought to increase the sensitivity of peptide detection and broaden the range of tissues covered through his analysis of chia proteomes. Targeted tissues were ungerminated seeds and the roots, hypocotyls, and cotyledons of five-day-old seedlings. As in Husselmann 2017, TCEP was employed for reducing disulfides, but iodoacetamide alkylated the cysteines. Mass spectrometry took place at the Mass Spectrometry and Proteomics Facility at the Johns Hopkins School of Medicine. The Thermo Orbitrap Fusion Lumos Tribrid mass spectrometer yielded an average of 54,868 tandem mass spectra per LC-MS/MS experiment, producing up to twenty MS/MS per second at its peak.
[doi:10.25345/C5Q79V]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: chia ; root ; hypocotyl ; cotyledon
Principal Investigators: (in alphabetical order) |
Bret Cooper, USDA-ARS, USA |
Submitting User: | dtabb73 |
Klein A, Husselmann LHH, Williams A, Bell L, Cooper B, Ragar B, Tabb DL.
Proteomic Identification and Meta-Analysis in Salvia hispanica RNA-Seq de novo Assemblies.
Plants (Basel). Epub 2021 Apr 14.
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