This DATASET collection includes the mass spectrometry files for proteomics venom investigation of seven taxa of the genera Vipera, Montivipera, Macrovipera and Daboia across Turkiye/Turkey.
Species list:
Vipera berus barani
Vipera darevskii
Montivipera bulgardaghica bulgardaghica
Montivipera bulgardaghica albizona
Montivipera xanthina
Macrovipera lebetinus obtusa
Daboia palaestinae
Folders 01-07 - BOTTOM-UP PROTEOMICS: The venom pools were investigated by the bottom-up "snake venomics" (labled as SVX) approach and in short: separated by RP-HPLC, followed by SDS-PAGE separation and the single bands were in-gel processed by DTT, IAC and finally o/n tryptic digested. Samples submitted to HPLC-MS/MS. Early peptidic fractions of the first HPLC run were directly submitted to HPLC-MS/MS analytic w/o further gel procession. Folders 01 to 07 include the MS and MS/MS spectra of the snake species 1-7, respectively. Files are included as RAW and MZML format.
Used instrument: LTQ Orbitrap XL mass spectrometer (Thermo, Bremen, Germany) with an Agilent 1260 HPLC system (Agilent Technologies, Waldbronn, Germany) using a reversed-phase Grace Vydac 218MS C18 (2.1 x 150 mm; 5 um particle size) column.
Modifications: UNIMOD:4 - \"Iodoacetamide derivative.\"
[doi:10.25345/C5Z02ZK9H]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: snake venomics ; venom ; snake ; viper ; snakebite ; proteomics ; peptidomics
Principal Investigators: (in alphabetical order) |
Maik Damm, TU Berlin, Deutschland |
Submitting User: | MDamm |
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Owner | Reanalyses | |
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Browse Dataset Files | |
FTP Download Link (click to copy):
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