MassIVE MSV000095637

Complete Public PXD054977

Extracellular proximity labeling_TIMP2 Interactome

Description

Assessment of the TIMP2 interactome in HT1080 and HS-5 cells using proximity labeling (TurboID and BioID2). File designations included in the excel spreadsheet. Metadata - file will be added when upload bugs are fixed. Comma separated metadata below: File Name Prefix,Cell Line,Biotin Ligase,Treatment,Label,x Identity / Notes, P1301-x-EXP_1,HT1080,TurboID,Untreated,N/A,1 = Control; 2 = TurboID; 3 = TIMP2-TurboID; 4 = TurboID-TIMP2, P1301-x-EXP_2,HT1080,TurboID,Untreated,N/A,5 = Control; 6 = TurboID; 7 = TIMP2-TurboID; 8 = TurboID-TIMP2, P1314-x-EXP_5,HT1080,TurboID,Concanavalin A treated,N/A,1 = Control; 2 = TurboID; 3 = TIMP2-TurboID; 4 = TurboID-TIMP2, P1314-x-EXP_6,HT1080,TurboID,Concanavalin A treated,N/A,5 = Control; 6 = TurboID; 7 = TIMP2-TurboID; 8 = TurboID-TIMP2, P1314-x-EXP_7,HT1080,BioID2,Untreated,N/A,9 = Control; 10 = BioID2; 11 = BioID2-TIMP2; 12 = TIMP2-BioID2, P1314-x-EXP_8,HT1080,BioID2,Untreated,N/A,13 = Control; 14 = BioID2; 15 = BioID2-TIMP2; 16 = TIMP2-BioID2, P1333-EXP_9-x,HT1080,TurboID,3D culture (spheroid),N/A,1 = Control; 2 = TurboID; 3 = TIMP2-TurboID; 4 = TurboID-TIMP2, P1407-x-EXP_21,HT1080,TurboID,3D culture (spheroid),N/A,1 = TurboID; 2 = TIMP2-TurboID; 3 = TurboID-TIMP2, P1333-EXP_10-x,HT1080,TurboID,PMA treated,N/A,1 = Control; 2 = TurboID; 3 = TIMP2-TurboID; 4 = TurboID-TIMP2, P1352-EX13-x,HT1080,TurboID,PMA treated,N/A,1 = Control; 2 = TurboID; 3 = TIMP2-TurboID; 4 = TurboID-TIMP2, P1358-1_126-129C,HS-5,TurboID,Untreated,TMT,126 = Control; 127C = TurboID; 128C = TIMP2-TurboID; 129C = TurboID-TIMP2, P1358-2_127N-130N,HS-5,TurboID,Untreated,TMT,127N = Control; 128N = TurboID; 129N = TIMP2-TurboID; 130N = TurboID-TIMP2 [doi:10.25345/C5NC5SQ5F] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: TIMP2 ; Proximity Labeling ; Interactomics

Contact

Principal Investigators:
(in alphabetical order)
David Peeney, National Cancer Institute, United States
Submitting User: dpeeney
Number of Files:
Total Size:
Spectra:
Subscribers:
 
Owner Reanalyses
Experimental Design
    Conditions:
    Biological Replicates:
    Technical Replicates:
 
Identification Results
    Proteins (Human, Remapped):
    Proteins (Reported):
    Peptides:
    Variant Peptides:
    PSMs:
 
Quantification Results
    Differential Proteins:
    Quantified Proteins:
 
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.