MassIVE MSV000088427

Complete Public PXD029870

Spatial Venomics - Cobra Venom System Reveals Spatial Distinction of Snake Toxins by Mass Spectrometry Imaging

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Description

Despite extensive research, there is still limited knowledge of the functional biology of most animal toxins, including their venom production and storage, as well as the morphological structures within sophisticated venom producing tissues that might underpin venom modulation. Here we applied non-targeted mass spectrometry imaging (MSI), in combination with standard proteomic and transcriptomic approaches, to enable discrete toxin mapping in high-resolution intensity maps across a snake venom gland sourced from the Egyptian cobra (Naja haje). Matrix-assisted laser desorption/ionization (MALDI) MSI toxin visualization on the elapid venom gland reveals surprising spatial heterogeneity of different toxin classes at the proteoform level, which may be the result of physiological constraints on venom production and/or storage, or reflect the potential for venom modulation under different stimuli. [doi:10.25345/C5JC55] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Spatial Venomics ; Mass Spectrometry Imaging ; Venom Gland Morphology ; Naja ; Venom Heterogeneity

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Principal Investigators:
(in alphabetical order) 		Benjamin-Florian Hempel, Charite - Universitaetsmedizin Berlin, Germany
Submitting User: benjamin_hempel
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