MassIVE MSV000094179

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The NAE1-mediated Neddylation operates as an essential post-translational modification checkpoint for effector CD8+ T cells

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Description

Cells were lysed with TFA, and precipitated with cold acetone. The precipitates were transferred to E3filters and digestion was performed overnight with trypsin. The peptides were desalted with StageTips, and were analyzed on Eclipse Orbitrap with DIA mode. For MS1, the resolution was set to 120,000. Mass range was 380-980 m/z. AGC target was standard. Max injection time was auto. For MS/MS, the isolation window was 8 m/z. Activation type was HCD with 30% NCE. The scan range was 145-1450 m/z. The normalized AGC target was 800%. Max injection time was auto. For FAIMS, three CVs were used, -40v, -55v, and -75v. [doi:10.25345/C58K7572P] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Quantitative proteomics ; Proteomics ; E3technology ; On-filter digestion ; NAE1/ULA1/Appbp1 ; Data-independent acquisition ; DIA

Contact

Principal Investigators:
(in alphabetical order) 		Gang Xin, The Ohio State University College of Medicine, USA
Submitting User: yayu

Publications

Jin J, Zhang R, Li J, Gao F, Liao Z, Yu Y, Wang Y, Bucci D, Xiao M, Ma R, Ma Q, Gao S, Lio J, Novais F, Huang SC, Zhu J, Ghoneim H, Wen H, Li Z, Sun N, Xin G.
The NAE1-mediated neddylation operates as an essential post-translational modification checkpoint for effector CD8+ T cells.
Proc Natl Acad Sci U S A. 2025 Mar 11;122(10):e2424061122. Epub 2025 Mar 3.

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