MassIVE MSV000097135

Partial Public

Coupled transcriptome and proteome analysis of the L3 and L4 developmental stages of Anisakis simplex s.s.: Insights into target genes under glucose influence

Subscribe Comment Reanalyze Spectra Add Reanalysis

Description

According to the European Food Safety Authority (EFSA), A. simplex has been classified as a biohazardous organism. Most developmental stages of parasitic nematodes occur under anaerobic conditions, and larvae obtain most of their energy from saccharides. The effect of glucose from the external environment has not been described in L3 and L4 larvae of A. simplex. Another advantage is that only these stages have been identified as pathogenic in humans. In this study, we aimed to uncover genes and pathways controlling glucose (10 mg/mL) action by an integrated analysis of the transcriptome and proteome of L3 and L4 larval stages of A. simplex. The proteome results yielded a total of 6,441 proteins that were filtered for further analysis. The final repository consists of 464 proteins, which were identified in a group as up-regulated DEPs, e.g., malate dehydrogenase, and down-regulated DEPs, e.g., methylmalonyl-CoA epimerase. The results obtained should lead to a better understanding of the molecular processes underlying the development of A. simplex infection in humans and will add to the existing knowledge on the role of nutrients of this parasite. [doi:10.25345/C5VD6PH4M] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Anisakis simplex ; glucose uptake ; proteomics ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Robert Stryinski, University of Warmia and Mazury in Olsztyn, Poland
Submitting User: robertstr
Number of Files:
Total Size:
Spectra:
Subscribers:
 
Owner Reanalyses
Experimental Design
    Conditions:
    Biological Replicates:
    Technical Replicates:
 
Identification Results
    Proteins (Human, Remapped):
    Proteins (Reported):
    Peptides:
    Variant Peptides:
    PSMs:
 
Quantification Results
    Differential Proteins:
    Quantified Proteins:
 
Browse Dataset Files
 
FTP Download Link (click to copy):

Species

Instrument

Modifications

- Dataset Reanalyses

+ Dataset History

Click here to queue conversion of this dataset's submitted spectrum files to open formats (e.g. mzML). This process may take some time.

When complete, the converted files will be available in the "ccms_peak" subdirectory of the dataset's FTP space (accessible via the "FTP Download" link to the right).
Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.