MassIVE MSV000089722

Partial Public PXD034877

Proteomic Characterization of Human Milk Fat Globule Membrane Proteins during a 12 Month Lactation Period

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Description

The milk fat globule membrane (MFGM) contains proteins which have been implicated in a variety of health benefits. Milk fat globule membrane proteins were isolated from human milk during a 12 month lactation period and subjected to in solution digestion and liquid chromatography tandem mass spectrometry analysis. Data were pooled, and our results showed that 191 proteins were identified. Relative quantification of the identified MFGM proteins during the course of lactation was performed by label free spectral counting and differentiation expression analysis, which showed some proteins decreasing during the course of lactation whereas some increased or remained at a relatively constant level. The human MFGM proteins are distributed between intracellular, extracellular, and membrane-associated proteins, and they are mainly involved in cell communication and signal transduction, immune function, metabolism and energy production. This study provides more insights into the dynamic composition of human MFGM proteins, which in turn will enhance our understanding of the physiological significance of MFGM proteins. [doi:10.25345/C5H12VC0J] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Lactation ; Human Milk ; MFGM

Contact

Principal Investigators:
(in alphabetical order) 		Bo Lonnerdal, Department of Nutrition University of California, Davis, Californi, USA
Submitting User: brettsp1

Publications

Yalin Liao, Rudy Alvarado, Brett Phinney, Bo Lönnerdal.
Proteomic characterization of human milk fat globule membrane proteins during a 12 month lactation period.
J Proteome Res. 2011 Aug 5;10(8):3530-41. doi: 10.1021/pr200149t. Epub 2011 Jun 29. PMID: 21714549.

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